Using ultra-pure β-carotene as the substrate, canthaxanthin is synthesized via high-specificity immobilized enzyme catalysis. The enzyme preparation can be reused, leading to a reduction of the production cycle by over 30%. The entire process generates no unnecessary impurities, thereby enabling higher separation & purification efficiency and ensuring a final product purity consistently above 99.0%. Compared to traditional direct fermentation processes, it offers significant cost advantages. Simultaneously, compliant chemical synthesis processes are implemented to precisely match regulatory standards across different regions, ensuring suitability for diverse application scenarios.